Electrochemical Immunosensor Modified with Nitrogen-Doped Reduced Graphene Oxide@Carboxylated Multi-Walled Carbon Nanotubes/Chitosan@Gold Nanoparticles for CA125 Detection

Lung cancer is one of the malignant tumors with highest mortality rate, and detection its tumor marker carcinoma antigen 125 (CA125) significant. Here, an electrochemical immunoassay for CA125 was described. Nitrogen-doped reduced graphene oxide (N-rGO), carboxylated multi-walled carbon nanotubes (CMWCNTs) gold nanoparticles (AuNPs) were applied to co-modify glassy electrode (GCE), after incubation Anti-CA125, modified employed specific CA125. The N-rGO@CMWCNTs (Nitrogen-doped oxide@carboxylated nanotubes) used as a matrix, while CS@AuNPs (Chitosan@gold nanoparticles) high conductivity biocompatibility immobilized on it through reaction between carboxyl groups from CMWCNTs amino groups, hydroxyl chitosan (CS), resulting in effect double signal amplification. immunosensor demonstrated excellent performance linear range 0.1 pg mL?1–100 ng mL?1, limit low 0.04 mL?1 (S/N = 3). It had been verified that this method good precision accuracy, could remain stable 10 days. This research provided new serum.